Figure 2 | Scientific Reports

Figure 2

From: Dimethyl fumarate blocks pro-inflammatory cytokine production via inhibition of TLR induced M1 and K63 ubiquitin chain formation

Figure 2

DMF inhibits LPS induced gene transcription.

BMDMs were incubated with 50 μM DMF for 4 h and then stimulated with LPS for 1 h as indicated. Total RNA was then isolated and the induction of TNF, IL-6, IL-10, GM-CSF, IL-12p40, IL-23p19, IκBα and IFNβ determined by qPCR as described in the methods. Error bars represent the standard deviation of 4 BMDM cultures. For a comparison of LPS stimulation with or without DMF treatment a p value (Students t-test) of <0.01 is indicated by ** and p < 0.001 by ***.

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