Figure 3 | Scientific Reports

Figure 3

From: Dimethyl fumarate blocks pro-inflammatory cytokine production via inhibition of TLR induced M1 and K63 ubiquitin chain formation

Figure 3

Effect of DMF on LPS stimulated mRNA induction in Nrf2 knockout BMDMs.

BMDMs were isolated from wild type and Nrf2 knockout mice. Cells were incubated with 50 μM DMF for 4 h and then stimulated with LPS for 1 h as indicated. Total RNA was then isolated and the induction of TNF, IL-6, IL-10, GM-CSF. IL-12p40, IL-23p19, IκBα and HO-1 determined by qPCR. Error bars represent the standard deviation of measurements from independent cultures from 4 mice per genotype. For a comparison of LPS stimulation in the presence and absence of DMF a p value (Students t-test) of <0.05 in indicated by *, p < 0.01 by ** and p < 0.001 by ***. For a comparison between wild type and Nrf2 knockout cells a p < 0.05 is indicated by # and p < 0.01 by ##.

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