Figure 4 | Scientific Reports

Figure 4

From: Dimethyl fumarate blocks pro-inflammatory cytokine production via inhibition of TLR induced M1 and K63 ubiquitin chain formation

Figure 4

Effect of DMF on LPS stimulated mRNA induction in MSK1/2 knockout BMDCs.

BMDCs were isolated from wild type and MSK1/2 double knockout mice. Cells were incubated with 50 μM DMF for 4 h and then stimulated with LPS for 1 h or 16 h as indicated. Total RNA was then isolated and the induction of IL-10, IL-6, GM-CSF, IL-12p35, IL-12p40 and IκBα was determined by qPCR. Error bars represent the standard deviation of measurements from independent cultures from 4 (0 and 1 h) or 3 (16 h) mice per genotype. A p value (Students t-test) between the presence and absence of DMF of less that 0.01 is indicated by ** and a p < 0.001 by ***. For a comparison of wild type and MSK1/2 knockout cells in the absence of DMF, p < 0.05 and p < 0.01 are indicated by # and ## respectively.

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