Figure 9 | Scientific Reports

Figure 9

From: Dimethyl fumarate blocks pro-inflammatory cytokine production via inhibition of TLR induced M1 and K63 ubiquitin chain formation

Figure 9

Effect of DMF on LPS stimulated polyubiquitin chain formation in BMDMs.

(A) BMDMs were incubated in the presence or absence of 50 μM DMF for 4 h. Cells were then either left unstimulated or treated with 100 ng/ml LPS for a further 30 min. Halo-NEMO beads were used to pulldown M1 polyubiquitin chains. Pull downs were then immunoblotted with antibodies specific for M1 chains or IRAK2 (left hand panels). Alternatively the levels of IRAK1, phospho p105, phospho ERK1/2 and total ERK1/2 in the cell extracts were determined by immunoblotting (right hand panels). (B) As (A) but Halo-TUBE pull downs were used to isolate total ubiquitin chains and the pull downs blotted with antibodies against M1 and K48 chains.

Back to article page