Figure 5: Deletion of calpains resulted in alterations in cell wall integrity. | Scientific Reports

Figure 5: Deletion of calpains resulted in alterations in cell wall integrity.

From: Calpains are involved in asexual and sexual development, cell wall integrity and pathogenicity of the rice blast fungus

Figure 5

(A) M. oryzae strains were incubated on CM plates supplemented with various stress inducers at 28 °C for 6 days. Growth of strains in media supplemented with SDS (0.1%), Congo red (200 mg/ml) and CFW (100 μg/ml). (B) Sensitivity of M. oryzae strains to cell-wall-digesting enzymes. Bars represent the mean number of protoplasts (×104/ml) released by the hyphae of mutants or Guy11 after a 30 min incubation with Glucanex solution. Error bars represent one standard deviation (P < 0.01). Different letters indicate a statistically significant difference. (C) Sensitivity of strains to cell-wall-digesting enzymes. The wild-type strain Guy11 and the mutants were photographed after treatment with Glucanex for 30 min. A large number of protoplasts were released from the mycelia of the ΔMocapn7, ΔMocapn9, and ΔMocapn14 mutants. Few protoplasts were released from the mycelia of the ΔMocapn9ΔMocapn7 and ΔMocapn4ΔMocapn7 mutants. Scale bar = 25 μm.

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