Figure 4: ARFRP1 is involved in the regulation of LD growth in HCV infected cells. | Scientific Reports

Figure 4: ARFRP1 is involved in the regulation of LD growth in HCV infected cells.

From: ADP-ribosylation Factor-related Protein 1 Interacts with NS5A and Regulates Hepatitis C Virus Propagation

Figure 4

(A) Huh7.5 cells were seeded on cover slides, transfected with either negative siRNA or ARFRP1-specific siRNA for 48 h, and then infected with Jc1. At 48 h postinfection, cells were fixed in 4% paraformaldehyde, and immunofluorescence staining was performed by using a rabbit anti-NS5A antibody and TRITC-conjugated donkey anti-rabbit IgG to detect NS5A (red). Cells were further incubated with 1 μM of BODIPY (439/503) (Invitrogen) to detect LDs (green). Dual staining showed colocalization of NS5A and LDs as yellow fluorescence in the merged image. The boxed area in the merged image is enlarged at the bottom. Cells were counterstained with DAPI to label nuclei (blue). (B) Huh7.5 cells were treated as described in (A) and total cell lysates were immunoblotted with the indicated antibodies.

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