Figure 2
Effect of EP peptides on VEGFR2 phosphorylation.
Western blot analysis performed using anti-phospho-Tyr antibody on cellular membranes of (A) PAEC overexpressing VEGFR2 stimulated with VEGF or with EP0-6 peptides (50 ng/mL each, 15 min at 37 °C) (western blot quantification expressed as fold increase respect to Ctr: VEGF 6.5 ± 1.3, EP0 0.7 ± 0.25, EP1 0.75 ± 0.09 EP2 0.5 ± 0.18, EP3 4.1 ± 1.3 EP4 0.7 ± 0.16, EP5 4.7 ± 1.2, EP6 4.8 ± 1.8, AcHn 0.9 ± 0.08, AcHx 4.7 ± 0.9) and (B) PAEC overexpressing VEGFR2 pretreated with 50 ng/mL of EP0-6 peptides for 15 min and then with 50 ng/mL of VEGF for additional 15 min in serum deprivation condition (Ctr). AcHn and AcHx peptides (50 ng/mL) and the tyrosine kinase inhibitor SU5614 (10 μM) were used as controls. Anti-VEGFR2 antibody was used as loading control. Gels are representative of three experiments (western blot quantification expressed as ∆ of fold increase respect to VEGF induced response: EP0 0.89 ± 0.08, EP1 1 ± 0.05, EP2 0.95 ± 0.04, EP3 0.76 ± 0.18, EP4 0.79 ± 0.23, EP5 1.12 ± 0.04, EP6 1.2 ± 1.3, SU5614 0.15 ± 0.05).
