Figure 3 | Scientific Reports

Figure 3

From: Cell-based phenotypic screening of mast cell degranulation unveils kinetic perturbations of agents targeting phosphorylation

Figure 3

Two Stat3 inhibitors (JSI124 and Stattic) display distinct TCRPs.

(a,b) Unlike for Stattic, the TCRP of JSI124 pretreated RBL-2H3 cells did not yield a typical inhibition curve. (a) IgE sensitized RBL-2H3 cells were pretreated with serial concentrations of JSI124 2 hours before DNP-BSA stimulation, cell status was monitored for 70 hours. (b) Sensitized cells were pretreated with indicated concentrations of Stattic before DNP-BSA stimulation as (a). (c,d) JSI124 and Stattic both inhibited Stat3 phosphorylation. (c) After sensitization, RBL-2H3 cells were treated with DNP-BSA (100 ng/mL) for indicated times. Whole cell extracts were subjected to immunoblotting with anti-p-Stat3, Stat3 and Actin antibodies, cell status was monitored for 60 hours. (d) Procedures are the same as in (c), except that mast cells were pretreated with 6 μM Stattic for 2 hours.

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