Figure 3 | Scientific Reports

Figure 3

From: CsPAO4 of Citrus sinensis functions in polyamine terminal catabolism and inhibits plant growth under salt stress

Figure 3

PAs substrate specificity of recombinant CsPAO4 and analysis of the CsPAO4-catalyzed reaction products of Spd and Spm.

(a) The enzymatic activity of recombinant CsPAO4 was determined in 100 mM phosphate buffer at pH 7.0 and 37 °C. (b) The enzymatic activity of recombinant CsPAO4 was determined in 100 mM phosphate buffer at pH 8.0 and 30 °C. (c,d) HPLC analysis of reaction products from Spd (c) and Spm (d), respectively. The top row shows the Put, Dap, Spd and Spm standard; the second and bottom rows show products analysis after incubation with CsPAO4 for 0, 30 and 60 min, respectively.

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