Table 1 Transposition of BACs in Rat Zygotes Mediated via Hyperactive piggyBac Transposase.
BAC DNA [ng/ul] | hyPBase [ng/ul] | Number of Embryos Injected | Number of Embryos Transferred | Number of Pups | % Number of Pups Relative to Number of Embryos Transferred | Number of Pups PCR Positive | % PCR Positive Pups Relative to Embryos Transferred | % PCR Positive Pups Relative to Total Number of Pups |
|---|---|---|---|---|---|---|---|---|
3 | 100 | 41 | 19 | 7 | 36.8% | 1 | 5.3% | 14.3% |
3 | 50 | 197 | 99 | 13 | 13.1% | 1 | 1.0% | 7.7% |
2 | 25 | 276 | 181 | 25 | 13.8% | 6 | 3.3% | 24.0% |
Cas9 [ng/ul] | ||||||||
2 | 25 | 124 | 78 | 15* | 32.1% | 1* | 1.3%* | 6.7% |
