Table 3 Targeting hSIRPa-BAC into the Rosa26 locus using CRISPR/Cas9 and TALENs.
BAC DNA | BAC [ng/ul] | sgRNA + Cas9 protein | TALENs | Number of Embryos Injected (%viable) | Number of Embros Transferred | Number of E15 Embryos (% Relative to Transferred) | Number of Embryos PCR Positive (% Relative to Total E15) | % NHEJ Positive (% Relative to Total E15) |
|---|---|---|---|---|---|---|---|---|
RP11-887J4 Rat-Rosa26−HAs with sgRNA Target Sequence | 2 | 3 uM + 3 uM | NA | 194 (76.3) | 148 | 60 (40.5) | 1 (1.7) | 22 (36.7) |
RP11-887J4 Rat-Rosa26−HAs without sgRNA Target Sequence | 2 | 3 uM + 3 uM | NA | 125 (69.6) | 87 | 28 (32.2) | 1 (3.6) | 12 (42.8) |
RP11-887J4 Rat-Rosa26−HAs with sgRNA Target Sequence | 2 | NA | 5 uM + 5 uM | X177 (68.9) | 122 | 39 (32.0) | 0 (0) | 9 (23.1) |
RP11-887J4 Rat-Rosa26−HAs without sgRNA Target Sequence | 2 | NA | 5 uM + 5 uM | 100 (66.0) | 66 | 13 (19.7) | 0 (0) | 4 (30.8) |
