Figure 4: Cardiac differentiation from Mesp1-CPCs into cardiac myocytes requires precise dosage and timing exposure to the growth factor BMP4. | Scientific Reports

Figure 4: Cardiac differentiation from Mesp1-CPCs into cardiac myocytes requires precise dosage and timing exposure to the growth factor BMP4.

From: Mesp1 Marked Cardiac Progenitor Cells Repair Infarcted Mouse Hearts

Figure 4

(A) The number of Meps1-EYFP+ cells were positively correlated to the time exposure to BMP4. FACS was performed on day 5 EB-derived cells. (B) BMP4 treatment led to precocious and augmented Mesp1 expression. N ≥ 3; *p < 0.05. (C) Prolonged exposure to BMP4 led to reduced expression of markers of the cardiac program. EBs were collected on day 8 and gene expression was analyzed by real-time RT-PCR. N ≥ 3; *p < 0.05.

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