Figure 1: Chemical structure of IMB5046 and its effect on microtubules. | Scientific Reports

Figure 1: Chemical structure of IMB5046 and its effect on microtubules.

From: A Novel Nitrobenzoate Microtubule Inhibitor that Overcomes Multidrug Resistance Exhibits Antitumor Activity

Figure 1

(a) Chemical structure of IMB5046. (b) Effect of IMB5046 on the cytoskeleton of NIH/3T3 cells. NIH/3T3 cells were incubated with or without 100 nM IMB5046 for 6 h. Microtubule was stained with anti-tubulin antibody and F-actin was stained with phalloidin-FITC. IMB5046 at 100 nM partially disrupted the microtubule structures characterized by short microtubule fragments, but had no effect on F-actin networks. Scale bar, 10 μm. (c) IMB5046 disrupted the microtubule structures in A431 cells. A431 cells were treated with 100 nM IMB5046, 500 nM paclitaxel or 100 ng/mL nocodazole for 6 h. Insets are mitotic spindles from the same preparation. Scale bar, 10 μm. (d) Microtubule assembly assay in A431 cells. IMB5046 increased the free tubulin content in a concentration-dependent manner. Representative result of three independent experiments is shown. The histogram shows the relative density of tubulin. Data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01 versus Ctrl. Ctrl, control; Noc, nocodazole; Col, colchcine; Ptx, paclitaxel.

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