Figure 3: IMB5046 binds to tubulin at the colchicine pocket. | Scientific Reports

Figure 3: IMB5046 binds to tubulin at the colchicine pocket.

From: A Novel Nitrobenzoate Microtubule Inhibitor that Overcomes Multidrug Resistance Exhibits Antitumor Activity

Figure 3

(a) Limited proteolysis assay. Tubulin treated with IMB5046 showed similar patterns of proteolysis with that of colchicine. Representative result of three independent experiments is shown. The histogram shows the relative density of each fragment. Data are expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.01 versus Ctrl. (b) Colchicine competition assay. IMB5046 and nocodazole decreased the intrinsic colchicine fluorescence, while vincristine did not. Data are expressed as mean ± SD (n = 3). (c) The superimposition of the predicted binding mode of colchicine (in stick, carbon in orange) with the crystal structure (in stick, carbon in grey), as well as the predicted binding mode of IMB5046 (in stick, carbon in purple). (d) The detailed interactions between IMB5046 and tubulin.

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