Figure 1
From: Efficient Genome Editing in Apple Using a CRISPR/Cas9 system
Schematic diagram of CRISPR/Cas9 target sites in the apple PDS gene and vector construction.
(a) Schematic position of four guide RNAs (gRNAs) targeting the apple PDS genome sequence. Boxes indicate exons; numbers indicate exon numbers; lines indicate introns. The sequence of each gRNAs is shown, the PAM sequences are surrounded by green boxes. (b) Schematic map of the CRISPR/Cas9 vector for plant stable transformation mediated by Agrobacterium. Numbers indicate the position in the vector, the right border is numbered 1–26.
