Figure 4: Hemolysis, cytotoxicity and cyclic-AMP accumulation upon α-MSH and designed analogues treatment.

In (a) concentration-dependent hemolysis induced upon 1 h incubation of mouse RBCs with peptides. In (b) % cytotoxicity against 3T3 murine fibroblast cell line at 50 μM peptide concentration. In (c) induced accumulation of cyclic-AMP in B16 melanocytes on exposure to different concentrations of α-MSH and KKK-MSH. Each assay was done in triplicate on three different days and each data point represents mean ± S.D.