Figure 1: Tg(CaMKII-hA2AR) rats overexpress hA2AR in forebrain areas. | Scientific Reports

Figure 1: Tg(CaMKII-hA2AR) rats overexpress hA2AR in forebrain areas.

From: The caffeine-binding adenosine A2A receptor induces age-like HPA-axis dysfunction by targeting glucocorticoid receptor function

Figure 1

(a) Construct used to generate Tg(CaMKII-hA2AR) rats. (b,c) Animals present an overexpression of total A2AR in the forebrain confirmed by qPCR and Western blotting. The endogenous (right axis) rA2AR mRNA levels were not modified in the hippocampus as assessed with specific rat A2Aprimers. (d) A2AR protein levels increase from 2 weeks old onwards in the hippocampus and (e) no changes were detected in adenosine A1R mRNA levels for 12–14 weeks old animals. Results were analysed using unpaired Student’s t-test for each gene/brain area, *P < 0.05 compared to WT.

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