Table 1 Primers used for genotyping and qPCR.
Primer | Target Gene | Organism | Forward Primer | Reverse Primer | Amplicon Size (bp) |
|---|---|---|---|---|---|
CypA | PPIA peptidylprolyl isomerase A (cyclophilin A) | rat, human, mouse | TATCTGCACTGCCAAGACTGAGTG | CTTCTTGCTGGTCTTGCCATTCC | 126 |
Rpl13A | Ribosomal protein L13A | rat, mouse | GGATCCCTCCACCCTATGACA | CTGGTACTTCCACCCGACCTC | 130 |
Pgk1 | Phosphoglycerate kinase 1 | rat | ATGCAAAGACTGGCCAAGCTAC | AGCCACAGCCTCAGCATATTTC | 103 |
hACTB | Human Actin-β | human | GGACTTCGAGCAAGAGATGG | AGCACTGTGTTGGCGTACAG | 233 |
A2AH | Human Adenosine A2A Receptor | human, rat | AACCTGCAGAACGTCAC | GTCACCAAGCCATTGTACCG | 245 |
A2A | Adenosine Receptor A2A | rat, mouse | ATTCCACTCCGGTACAATGG | AGTTGTTCCAGCCCAGCAT | 115 |
A1 | Adenosine A1 Receptor | rat | ACCTCCGAGTCAAGATCCCT | TTGGCTCTCCAGTCTTGCTC | 160 |
Act-B | Actin-β | rat | AGCCATGTACGTAGCCAT | CTCTCAGCTGTGGTGGTGAA | 228 |
CaMKII-hA2A | calmodulin-dependent protein kinase II promoter and human Adenosine Receptor A2A | transgene | GACTAAGTTTGTTCGCATCCC | GTGACACCACAAAGTAGTTGG | 450 |
GILZ | Glucocorticoid induced Leucine Ziper | rat, mouse | AGCAGCCACTCAAACCAACC | AACGGAAACCACATCCCCTC | 151 |
Bcl2 | B-Cell Lynphoma | rat, mouse | CTGGTGGACAACATCGCTCTG | GGTCTGCTGACCTCACTTGTG | 228 |
PER1 | Period 1 | rat, mouse | TCTCACAGTTCATCTTCTGGC | CTGTGAGTTTGTACTCTTGCTG | 81 |
CRH | Corticotropin-releasing hormone (CRH) | rat | TGCCAAGGGAGGAGAAGAGAGCG | GGGCCCTGCAAGGCAGACAG | 105 |
