Figure 5: Treatment with miR-214 inhibitor attenuated glomerular hypertrophy, accompanied by restoration of PTEN expression in db/db mice.
From: Cross talk between miR-214 and PTEN attenuates glomerular hypertrophy under diabetic conditions
(A) Representative immunohistochemical staining for PTEN in glomeruli (original magnification ×400).Scale bars: 20 μm. (B) The mRNA levels of miR-214, PTEN, α-SMA and SM22 in isolated glomeruli detected by quantitative real-time PCR. (C) The protein levels of PTEN, α-SMA, SM22 and Collagen IV in isolated glomeruli detected Western blot. (D) Quantitative analysis of the protein levels of PTEN, α-SMA, SM22 and Collagen IV. db/m, db/m control mice; db/db, diabetic db/db mice; ScRNA, db/db mice treated with miR-214 scramble; anti-miR-214, db/db mice treated with miR-214 inhibitor. All data were expressed as means ± SD, ▲p < 0.05 vs. db/db group, ★p < 0.05 vs. ScRNA group.
