Figure 5: The role of NOX5-S in TDCA-induced DNA damage. | Scientific Reports

Figure 5: The role of NOX5-S in TDCA-induced DNA damage.

From: Bile acid receptor TGR5, NADPH Oxidase NOX5-S and CREB Mediate Bile Acid-Induced DNA Damage In Barrett’s Esophageal Adenocarcinoma Cells

Figure 5

(A) Knockdown of NOX5-S significantly decreased the tail moment in response to TDCA treatment in FLO-1 cells (N = 130–171 cells of 3 experiments). (B) Representative images of the Comet Assay at 4X magnification in FLO-1 cells transfected with pCMV or NOX5-S plasmid with or without TDCA treatment (10−11 M, 24 hours). (C) Overexpression of NOX5-S significantly increased the tail moment in response to TDCA treatment in FLO-1 cells (N = 136–172 cells of 3 experiments). These data suggest that NOX5-S may mediate TDCA-induced DNA damage in FLO-1 cells. ANOVA **P < 0.01, compared with Control siRNA group or pCMV group; #P < 0.05, compared with Control siRNA group plus TDCA group or pCMV plus TDCA group.

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