Figure 8: The role of CREB in TDCA-induced DNA damage.

(A) Knockdown of CREB significantly decreased the tail moment in response to TDCA treatment in FLO-1 cells (N = 187–308 cells of 3 experiments). (B) Overexpression of CREB significantly increased the tail moment in response to TDCA treatment in FLO-1 cells (N = 171–369 cells of 3 experiments). These data suggest that CREB may mediate TDCA-induced DNA damage in FLO-1 cells. ANOVA **P < 0.0001, compared with Control siRNA group or pCDNA group; ##P < 0.001, compared with Control siRNA group plus TDCA group or pCDNA plus TDCA group.