Figure 1: miR-125b is involved in the GnRH induction of LH and FSH expression.
From: A regulatory loop between miR-132 and miR-125b involved in gonadotrope cells desensitization to GnRH
(a,b) Rat primary pituitary cells were electroporated with a miR-125b expressing vector or an empty vector and then treated with 1 nM GnRHa for 4 hours. (a) The concentration of accumulated LH and FSH into the medium was measured by ELISA. Overexpressing miR-125b significantly reduced basal secretion of LH and prevented the GnRHa-induced secretion of LH and FSH (n = 4). (b) Lhb and Fshb mRNA levels were measured by qRT-PCR and normalized to Gapdh mRNA. Overexpressing miR-125b nearly abolished basal expression of both Lhb and Fshb mRNA and prevented their GnRH-induced expression (n = 3). (c) LβT2 cells were electroporated with a miR-125b expressing vector or an empty vector and then treated with 10 nM GnRHa for 4 h. Overexpression of miR125 prevented the GnRHa-increased Lhb and Fshb mRNA expression (n = 7). (d) LβT2 cells were electroporated with anti-miR-125b or scrambled LNA. Blocking miR-125b increased both LHb and Fshb mRNA levels (n ≥ 10). Different letters illustrate significant differences. *P < 0.05; **P < 0.01; ***P < 0.001.
