Figure 1
From: Transgenic overexpression of VEGF-C induces weight gain and insulin resistance in mice
1 Increased weight gain and adipose tissue accumulation in K14-VEGF-C mice.
(a) ELISA analysis revealed increased murine VEGF-C levels in SWAT of obese mice that were kept under HFD for 20 weeks. (b) Immunofluorescence analysis of tail skin showing enlarged lymphatic vessels (podoplanin, Pdpn, green) in K14-VEGF-C mice, while whole-mount immunostains of intestinal villi showed no differences in lacteal (LYVE-1, green) or blood vessel (CD31, red) structure between WT and K14-VEGF-C mice. (c) K14-VEGF-C mice gained more weight than WT littermates (n = 8 per group, mean ± SEM is shown, *p < 0.05 and **p < 0.01 compared to WT control at the same time point with a two-tailed Student’s t-test; effect of genotype: p < 0.0001 with a two-way ANOVA) and (d) had increased % of SWAT, but not EWAT. (e,f) K14-VEGF-C mice had larger adipocytes in SWAT. Scale bars = 100 μm. **p < 0.01, ***p < 0.001, two-tailed Student’s t-test. Data are mean ± SD.
