Figure 2
Effects of peptide concentration and CD80 costimulation.
(a + b) Art v 1 specific (left) and Bet v 1 specific (right) T cell reporter cells were cocultured with CD80− eAPC (a) or CD80+ eAPC (b) expressing the appropriate restriction elements in the presence of allergenic peptides used at the indicated concentrations. Mean gMFI ± SD of triplicates is shown. (c) Effect of CD80 costimulation on Art v 1 and Bet v 1 reporter activation using Art v 123–36 or Bet v 1142–153 peptide at the indicated concentrations. Mean fold induction is shown for duplicate values and median fold induction of all experiments is indicated as line. Statistics by two-way ANOVA, followed by Bonferroni post-test (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ns not significant).
