Figure 3
Comparison of endogenous loading strategies: epitope variation, Ii chain and LAMP-1.
(a) Schematic illustration of fusion proteins of the invariant chain and allergenic peptides. (b) Comparison of exogenous and endogenous MHCII loading strategies via addition of allergenic peptides or expression of invariant chain (Ii) fusion proteins in the eAPC, respectively. 5 μg/mL Art v 123–36 and 0.5 μg/mL Bet v 1142–153 peptide were used were used for exogenous loading. Mean fold induction is shown for duplicate values and median fold induction of all experiments is indicated as line. Statistics by one-way ANOVA, followed by Tukey’s multiple comparison post-test (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ns not significant). (c) Schematic illustration of fusion proteins of LAMP-1 (lysosomal-associated membrane protein 1) and allergenic peptides. (d) Comparison of exogenous and endogenous MHCII loading strategies via peptide addition and Ii or LAMP1 fusion proteins. 5 μg/mL Art v 123–36 and 0.5 Bet v 1142–153 peptide were used for exogenous loading. Mean fold induction is shown for duplicate values and median fold induction of all experiments is indicated as line. Statistics by one-way ANOVA, followed by Tukey’s multiple comparison post-test (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ns not significant); (TM: transmembrane domain).
