Figure 4
Competition for MHCII loading.
(a) Art v 1 reporter T cells were stimulated with Art v 123–36 peptide alone or in presence of indicated amounts of competitor peptide (HA306–318 peptide). Inhibition of stimulation is shown for duplicate values and median inhibition of five experiments is indicated as line. Statistics by one-way ANOVA, followed by Tukey’s multiple comparison post-test (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ns not significant). (b) Effect of HA306–318 peptide addition as competitor for the presentation of endogenously loaded allergenic Art v 1 peptide. Inhibition of stimulation is shown for duplicate values and median inhibition of five experiments is indicated as line. Statistics by one-way ANOVA, followed by Tukey’s multiple comparison post-test (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ns not significant). (c) Art v 1 reporter cells were stimulated with Art v 1 peptide in the presence of eAPC endogenously expressing competitor peptide (Ii::HA306–318) or control eAPC. Mean fold induction of duplicates ± SD of five experiments is shown. Statistics by two-way ANOVA, followed by Bonferroni post-test (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ns not significant).
