Figure 6

Proliferation of allergen-specific T cells in response to engineered APCs.

(a) A CFSE-labeled Art v 1-specific T cell clone was cocultured with eAPC peptide expressing HLA-DRB1 and CD80. eAPC endogenously loaded via invariant chain fusion proteins with Art v 1_25–34 or unloaded eAPC were used and T cells cultured without stimulus were used as additional control. After 4 days of coculture cells were harvested, stained for CD4 expression and analyzed by flow cytometry. Data is representative for six tested Art v 1-specific TCC. (b) A CFSE-labeled Bet v 1-specific T cell line (derived from a HLA-DRB1:01⁺ and HLA-DRB1:03⁺ donor) was cocultured with eAPC peptide expressing restriction elements and CD80 as indicated. eAPC preloaded with Bet v 1_142–153 or unloaded APC were used and T cells cultured without stimulus were used as additional control. Following 5 days of coculture cells were harvested, stained for CD4 expression and analyzed by flow cytometry. Cell population in the CFSE^lowCD4⁻ gate represent eAPCs. (c) Mean and SD of CFSE^lowCD4⁺ T cells from duplicate wells of (b) are shown. The experiment was repeated with similar outcome.