Table 2 Bacterial strains and plasmids used in this study.
Designation | relevant characteristics | reference |
|---|---|---|
Salmonella enterica serovar Typhimurium strains | ||
NCTC12023 | wild type | lab stock |
LT2 | wild type | lab stock |
MvP812 | ΔsiiF::FRT | |
MvP818 | ΔinvC::FRT | |
MvP850 | ΔhilA::FRT | |
MvP1830 | ΔfliM::FRT | this study |
MvP1729 | tsr::I-SceI cat | this study |
MvP1813 | siiF::HaloTag aph | this study |
MvP1814 | spaS::HaloTag aph | this study |
MvP1816 | spaS::SNAP-tag aph | this study |
MvP1817 | tsr::SNAP-tag aph | this study |
MvP1859 | siiF::HaloTag FRT | this study |
MvP1860 | spaS::HaloTag FRT | this study |
MvP1909 | hilA::HaloTag aph | this study |
EM1079 | fliM::HaloTag | this study |
EM1080 | fliN::SNAP-tag | this study |
EM1081 | fliN::HaloTag | this study |
MvP1923 | spaS::HaloTag aph fliN::SNAP-tag FRT | this study |
MvP1926 | spaS::SNAP-tag aph fliN::HaloTag FRT | this study |
Plasmids | ||
pKD46 | Red-expressing vector, ts, AmpR | |
pCP20 | FLP-expressing vector, ts, AmpR | |
p2795 | core template plasmid, aph, AmpR | |
p3223 | pWSK29 PsiiA::siiF | |
p3545 | pWSK29 PinvF::invC | this study |
p3719 | pWSK30 L16-HaloTag | this study |
p3718 | pWSK30 L16-SNAP-tag | this study |
p3723 | PinvF::invC::L16-SNAP-tag | this study |
p3724 | PsiiA::siiF::L16-HaloTag | this study |
p3725 | PinvF::invC::L16-HaloTag | this study |
p3779 | SNAP-tag aph | this study |
p3780 | HaloTag aph | this study |
p3795 | pWSK29 PsiiA ::siiC | this study |
p3861 | PsiiA ::siiC::L16-SNAP-tag | this study |
p3866 | PsiiA ::siiC::L16-HaloTag | this study |
p4592 | PinvF ::SNAP-tag | this study |
p4594 | PinvF ::HaloTag | this study |
