Figure 2 | Scientific Reports

Figure 2

From: Structurally novel steroidal spirooxindole by241 potently inhibits tumor growth mainly through ROS-mediated mechanisms

Figure 2

Antiproliferative effects of by241 on selected human cell lines.

(A) and (B) Human cells were treated with different concentrations of by241 for 72 h. Cell viability determined by MTT assay; (C) Representative images of MGC-803 cells colonies after treatment with various concentrations (0.31, 0.62 or 1.25 μM) of by241 for 12 days; (D) Quantitative analysis of the colony formation inhibition rate in Fig. 1C. Apoptosis related morphology and nuclear condensation of MGC-803 (E,F) induced by by241. Apoptosis related morphology and nuclear condensation of EC9706 (G,H) induced by by241. Cells were cultured with different concentrations of by241 (2.5, 5 and 10 μM) for 24 h and detected using the Hoechst 33258 assay. Data are presented as the mean  ±  SD of three independent experiments. **P < 0.01 was considered statistically compared to control.

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