Figure 4
The role of ROS in by241-induced cancer cells death.
After pretreatment with or without 5 mM NAC for 2 hours, MGC-803 cells were treated with or without by241 for 24 hours, then the apoptosis related morphology (A) and the intracellular ROS was detected by fluorescence microscope (B); After pretreatment with or without 5 mM NAC for 2 hours, MGC-803 cells (C) or EC9706 cells (E) were treated with or without by241 for 24 hours, then the intracellular ROS was detected by flow cytometry; (D,F) Statistical analysis of ROS levels of (C,E); (G) After pretreatment with or without 2 mM or 5 mM NAC for 2 hours, MGC-803 cells were treated with or without by241(6.25, 12.5 μM) for 24 hours, cell viability were detected by the MTT assay. Data are presented as the mean ± SD of three independent experiments. **P < 0.01 and ##P < 0.01 were considered statistically compared to corresponding control. *P < 0.05, **P < 0.01 compared to control; ##P < 0.01 NAC+ by241-5 μM compared to by241–5 μM or NAC+ by241–10 μM compared to by241–10 μM.
