Figure 6
Expression changes of apoptosis related proteins induced by by241.
(A) By241 induced expression changes of Bcl-2 family (Bcl-2, Mcl-1, Bcl-XL, Bak, Bid, Bax) proteins; (B–G) Statistical analysis of Bcl-2 family (Bcl-2, Mcl-1, Bcl-XL, Bak, Bid, Bax) proteins expression change induced by by241; (H) By241 induced caspase-3 and caspase-9 activation; (I–L) Statistical analysis of pro-caspase-3, cleaved caspase-3, pro-caspase-9 and cleaved caspase-9 expression levels; (M–P) By241 did not change the expression of TRL, DR5 and cleaved caspase-8. (Q) Effect of pan caspase inhibitor Z-VAD-FMK significantly attenuated by241-induced apoptosis MGC-803 cells; (R) Statistically analysis of the FITC-positive cells. Data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 compared to control; ##P < 0.01 Z-VAD-FMK+by241-5 μM compared to by241-5 μM.
