Figure 5
From: SGO1 is involved in the DNA damage response in MYCN-amplified neuroblastoma cells
SGO1 knockdown together with MYCN overexpression induces senescence-like phenotype, but not apoptosis.
(a) TUNEL staining of control or SGO1-knockdown IMR32 cells 6 days after lentivirus infection. (b) MYCN-overexpressing or control SH-EP cells infected with lentiviral vectors expressing shRNA against SGO1 were assayed for SA-β-gal activity 6 days after infection. (c) The percentage of SA-β-gal-positive cells shown in (b). Values represent the mean ± SE of three fields. (d) Relative expression of p16 and p21 in the cells shown in (b). Values are expressed as the mean ± SE.
