Figure 3 | Scientific Reports

Figure 3

From: 15-Lipoxygenase metabolites of α-linolenic acid, [13-(S)-HPOTrE and 13-(S)-HOTrE], mediate anti-inflammatory effects by inactivating NLRP3 inflammasome

Figure 3

ALA metabolites, 13-(S)-HPOTrE and 13-(S)-HOTrE, inhibit inflammasome complex in RAW 264.7 cells.

(A) In semi-quantitative PCR analysis, 13-(S)-HPOTrE and 13-(S)-HOTrE showed decrease in transcript level of NLRP3, caspase-1, IL-1β and IL-18 at various concentrations (1, 50 and 100 μM) pre-incubated for 3 h then stimulated with LPS for 24 h. GAPDH was used as an internal control. (B) Estimation of IL-1β levels in the culture medium of cells as per the treatment described in (A). Dexamethasone (10 μM) was used as positive control. The values represent mean ± SD of three independent experiments. *Indicates significance (p < 0.05) compared to LPS alone treated cells. (C) Immunofluorescence microscopy of RAW 264.7 cells pre-treated with ALA metabolites (100 μM) for 3 h then stimulated with LPS for 24 h. Immunostained for NLRP3 (Green), caspase-1 (Red) and DAPI (Blue). Bars Scale: 10 μm. Data show representative of three independent experiments.

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