Figure 4
From: Up- regulation of miR-328-3p sensitizes non-small cell lung cancer to radiotherapy
Identification of miR-328-3p directly to the target gene and its influence.
(A) miR-328-3p binding to its site in the target gene H2AX. (B) Comparison of luciferase activity in vectors containing H2AX wild or mutation binding sequences. (C) Transient over expression of H2AX reversed the radiation sensitizing effect of miR-328-3p mimic in A549 cells with irradiation treatments. *p < 0.05. (D) Western blot analysis of DSB marker proteins γ-H2AX, H2AX, 53BP1 and RAD51 in A549 cells with miR-328-3p in transfected and non-transfected cells before and after exposure to 8-Gy X-ray irradiation. β-actin used as an internal control. Each experiment was performed in triplicate. Relative expression level demonstrated as gel images below.
