Figure 2 | Scientific Reports

Figure 2

From: MPT0G066, a novel anti-mitotic drug, induces JNK-independent mitotic arrest, JNK-mediated apoptosis and potentiates antineoplastic effect of cisplatin in ovarian cancer

Figure 2

MPT0G066 increases phosphorylation of JNK and alters the regulatory proteins of G2/M transition and Bcl-2 protein family.

SKOV3 or A2780 cells were treated with the various concentrations of MPT0G066 (0–1 μM) for 18 h. The whole cell lysates of SKOV3 or A2780 were subject to western blot analysis with (A) phosphorylated JNK (Thr-183 and Tyr-185), (B) MPM-2, cyclin B1, total or phosphorylated cdc2 (Tyr-15 and Thr-161), total or phosphorylated cdc25c (Ser-216), aurora A, aurora B, total or phosphorylated PLK-1 (Thr-210), to phosphorylated H3 (Ser-10), (C) total or phosphorylated Bcl-2 (Ser-70), Bax and Bim. Actin was served as a loading control.

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