Figure 6
From: Neuregulin 1 improves cognitive deficits and neuropathology in an Alzheimer’s disease model
Type I and type III NRG1 increase expression of Neprilysin in mice and in vitro.
Representative immunostaining for NEP (A) on sections of the hippocampus of non-Tg and APP-Tg injected with LV-control, LV-NRG1/I or LV-NRG1/III. (B) The area of neuropil occupied by NEP-immunoreactivity was calculated from 9 randomly selected sections per mouse (N = 3). (C) Western blotting analysis of NEP expression in primary hippocampal neuron cultures treated with 50 ng/ml recombinant soluble type I NRG1 and soluble type III NRG1, respectively. β-actin was used for loading control. Normalized levels of NEP show a marked increase of NEP by NRG1/I and NRG1/III. The results were expressed as mean ± SEM. **p < 0.01;***p < 0.0005.
