Figure 6: Subcellular localization of RAPTOR1B and SnRK1α1. | Scientific Reports

Figure 6: Subcellular localization of RAPTOR1B and SnRK1α1.

From: Quantitative phosphoproteomics reveals the role of the AMPK plant ortholog SnRK1 as a metabolic master regulator under energy deprivation

Figure 6

(a) Cytoplasmic localization of RAPTOR1B; z-stack maximum intensity projections of the signals originating from RAPTOR1B-YFP (green) and SnRK1α1-mCherry (magenta) transiently expressed in tobacco epidermal leaves. Stack dimensions x: 246.03 μm, y: 246.03 μm, z: 23.12 μm (40 frames). Nuclei are marked by arrows. (b) Diameter intensity blots over the nuclear area of maximum intensity projections from (a). Each blot is numbered and refers to the nuclei in (a). Concave curve shapes indicate localization of SnRK1α1-mCherry in the nucleus whereas convex curve shapes around the nuclear area indicate only cytoplasmic localization of RAPTOR1B-YFP. (c) Surface intensity blots of nuclear areas indicated in (a). (d) Co-localization of RAPTOR1B and SnRK1α1 in the cytoplasm. Overlay image of bright-field and signals from plastids (autofluorescence, red), SnRK1α1-mCherry (magenta) and RAPTOR1B-YFP (green) both transiently expressed in tobacco leaf epidermal cells. Rectangles indicate magnified areas of the image. Each magnified area contains separated RAPTOR1B-YFP and SnRK1α1-mCherry signals from the same image position.

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