Figure 7: SnRK1α1/RAPTOR1B in vivo interaction in planta by the BiFC assay.
Leaves of N. benthamiana were transiently transformed with mixtures of protein pairs using Agrobacterium-infiltration. Bait and prey proteins were fused to the N- and C-terminal part of Venus, respectively. Fluorescence and localization were observed by confocal laser scanning microscopy 48 h after infiltration. Bar represents 20 μm. The cytosolic FBPase fused to the C terminus of Venus expressed in combination with RAPTOR1b-VenusN173 induces no fluorescence signal and serves as a negative control (lower panel). All pictures were recorded with the same microscope settings. The experiment was repeated three times with similar results.
