Figure 2: Specific binding of anti-MUC1 antibodies to tumor but not normal cells and tissues. | Scientific Reports

Figure 2: Specific binding of anti-MUC1 antibodies to tumor but not normal cells and tissues.

From: Antibodies elicited by the first non-viral prophylactic cancer vaccine show tumor-specificity and immunotherapeutic potential

Figure 2

(a) Cell lines were stained with the indicated human anti-MUC1 antibodies. Mouse antibodies known to be specific for either the hypo-glycosylated MUC1 (4H5) or all MUC1 (3C6) and a human IgG isotype control antibody were used as positive and negative controls. HEK293T cells do not express MUC1 and are used as a negative control. MCF10A is an immortalized but not transformed cell line that expresses only fully glycosylated (normal) MUC1. Isotype is shown in gray and MUC1 staining in black. (b) Paraffin-embedded normal and tumor tissues from human colon, breast, lung and pancreas were stained with mouse antibody HMPV showing total MUC1 expression, with human IgG isotype control antibody as a negative control, and with antibodies H15K6 and H4K11. Images were taken at 20X magnification.

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