Table 1 CDR3 sequences, binding kinetics and predicted minimal epitope of human anti-MUC1 antibodies.
mAb | CDR3-H | CDR3-L | 25mer Affinity–Whole IgG | 100mer Affinity–Fab | Predicted Epitopea | ||||
|---|---|---|---|---|---|---|---|---|---|
ka (M−1 s−1) | kd (s−1) | KD (M) | ka (M−1 s−1) | kd (s−1) | KD (M) | ||||
H4K10 | LPESGGYSGWFDP | QQYYNTPFT | No Binding | Steady State* | 5.31 × 10−6 | APPHGVTS | |||
H4K11 | LPESGGYSGWFDP | QQYYSTPFT | No Binding | Steady State* | 1.57 × 10−5 | APPHGVTS | |||
H7L1 | GVVEVRGNHYYYYYMDV | QSYDSSLSGSGV | No Binding | 1.01 × 106 | 3.33 × 10−3 | 3.31 × 10−9 | APPHGVTS | ||
H9K4 | GSTNTYYYDSSGHP | MQALQTPQT | No Binding | 1.02 × 104 | 2.99 × 10 4 | 2.92 × 10−10 | APPHGVTS | ||
H14K6 | ERIGYCSGGSCYYYYYMDV | MQATQFPLT | 4.53 × 104 | 1.23 × 10−3 | 2.72 × 10−8 | 2.67 × 106 | 3.46 × 10−4 | 1.30 × 10−10 | PD T RP |
H15K6 | ENLGYCTGGNCFYYYYMDV | MQATQFPLT | 6.81 × 104 | 4.89 × 10−4 | 7.19 × 10−9 | 2.04 × 106 | 5.66 × 10−4 | 2.77 × 10−10 | PD T RP |
H16K6 | ENIGYCSGGSCFYYYYMDV | MQATQFPLT | 6.87 × 104 | 2.35 × 10−3 | 3.42 × 10−8 | 2.02 × 106 | 6.41 × 10−4 | 3.17 × 10−10 | PD T RP |
H16K16 | ENIGYCSGGSCFYYYYMDV | TQATQFPLT | 7.47 × 104 | koff (Too Fast) | — | 1.86 × 106 | 4.07 × 10−3 | 2.19 × 10−9 | PD T RP |
H17K7 | ELAD | MQGTHWPWT | 8.81 × 103 | koff (Too Fast) | — | 1.43 × 106 | 1.54 × 10−3 | 1.08 × 10−9 | GS T APP |
H19K6 | EDIGYCSGGSCFYYYYMDV | MQATQFPLT | 1.05 × 106 | koff (Too Fast) | — | 1.15 × 106 | 3.52 × 10−4 | 3.07 × 10−10 | PD T RP |
H19K15 | EDIGYCSGGSCFYYYYMDV | TQATQFPIT | 8.60 × 103 | koff (Too Fast) | — | 8.17 × 105 | 4.53 × 10−4 | 5.55 × 10−10 | PD T RP |
H21K7 | DQEHYFDGSGYYVDY | MQGTHWPWT | 1.32 × 104 | koff (Too Fast) | — | 3.07 × 106 | 6.62 × 10−3 | 2.16 × 10−9 | GS T APP |
H22K7 | DHDPDYGGYYMDV | MQGTHWPWT | 1.27 × 103 | koff (Too Fast) | — | 4.89 × 105 | 2.73 × 10−3 | 5.57 × 10−9 | GS T APP |
