Figure 2: Real-time quantitative RT-PCR analysis of OsGas2 expression profiles in rice suspension cells under different stresses. | Scientific Reports

Figure 2: Real-time quantitative RT-PCR analysis of OsGas2 expression profiles in rice suspension cells under different stresses.

From: Glucosidase II β-subunit, a novel substrate for caspase-3-like activity in rice, plays as a molecular switch between autophagy and programmed cell death

Figure 2

(a) DTT induced the expression of OsbZIP50 in wild type and OsGas2 RNAi cell lines. Protoplasts were prepared from suspension cells, and treated with 6.4 mM DTT for 18 h at 26–28 °C. (b) OsGAS2 expression in the protoplasts from suspension cells with the treatment of 6.4 mM DTT for different times. (c–e) OsGAS2 expression in 8-day-old seedling roots with the treatment of 4 °C (c), 150 mM NaCl (d), and 40% PEG 6000 (e) for different times. Total RNA was isolated for RT-PCR, and experiments were repeated three times.

Back to article page