Figure 2 | Scientific Reports

Figure 2

From: Development of a Functional Glomerulus at the Organ Level on a Chip to Mimic Hypertensive Nephropathy

Figure 2

Rationale and applicability of the physiologic GC.

(A) After 72 h of co-culture in the GC, a single layer of the glomerular endothelium (endothelium stained with CellTracker Red™, Thermo Scientific) closely apposed to a monolayer of podocytes (podocytes stained with CellTracker Green, Thermo Scientific), both of which were separated by a thin, extracellular membrane-coated membrane. (B) Immunostaining of endothelium CD-31 (red) and podocyte (green) synaptopodin after 3 days of co-culture. (C) Representative phase-contrast images do not show different morphology between cells in the transwell and cells in the GC. All images are x400 magnification. (D) Quantification of apoptosis in the endothelium and podocyte show no differences in the microfluidic device and in the transwell. (E) GFB permeabilities obtained by measuring the rate of transport of FITC-inulin, FITC-BSA and FITC-IgG are significantly reduced in co-cultures compared with BME-coated only and monocultures of the endothelium or podocytes under a perfusion flow rate of 5 μL/min. Data are the mean ± SD from three separate experiments. *P < 0.05, **P < 0.01.

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