Figure 4
Comparison of ROS accumulation and enzymatic activity in transgenic and wild-type plants under normal and cold stress conditions.
(a) DAB staining of H2O2 accumulation in leaves. Comparison of SOD (b), CAT (c), APX (d) and POD (e) activities in the leaves of transgenic and wild-type plants under normal and cold stress conditions. Six-week-old seedlings of transgenic lines (T2 generation) and wild-type were treated under cold 4 or 25 °C (control) for 3 d. For staining fully expanded terminal leaflet of upper leaves were detached from transgenic and wild-type plants. Leaves dark brown regions shown H2O2 generation. The samples shown are representative of six replicates. Values in (b–e) represent the means ± SE of three biological replicate samples. Each replicate sample was composite of leaves from twelve seedlings. C39, C65 and C75 represent three independent ShCML44 transgenic tomato lines. WT represents the wild-type AC. Asterisks indicate significant differences between transgenic lines and wild-type. *P < 0.05; **P < 0.01, Dunnett’s multiple comparisons test.
