Figure 2: SlMSI1 affects fruit ripening in tomato. | Scientific Reports

Figure 2: SlMSI1 affects fruit ripening in tomato.

From: Polycomb-group protein SlMSI1 represses the expression of fruit-ripening genes to prolong shelf life in tomato

Figure 2

(A) SlMSI1 transcript levels and protein abundance in the control and 3 overexpression transgenic lines. RNA and protein were extracted from tomato leaves. Overexpression lines L1, L2 and L29 contained 35S::SlMSI1-GFP. Transgenic plants carrying empty vector (35S::GFP) were used as the control. (B) Abnormal flowers of overexpression transgenic plants. (C) Dissections of abnormal flowers. (D) Changes in fruit ripening in the control, mutant rin and three overexpression lines at different stages. (E) SlMSI1 transcript levels and protein abundance in the control and suppression transgenic lines. RNA and protein were extracted from tomato leaves. Suppression lines SL1, SL2 and SL3 contained a 35S-driven SlMSI1 antisense cDNA fragment. SlMSI1 RNA and protein samples were derived from the same experiment, and electrophoretic gels and protein blots were processed in parallel. (F) Ripening comparison among the control and SlMSI1 suppression lines. (G) Production of ethylene in the control and SlMSI1 overexpression lines. Fresh fruits from different days after the breaker stage were sealed in airtight vials, and 1 ml of gas was sampled from the headspace after 24 h. DAB, days after breaker.

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