Figure 5: Genetic manipulation of SlMSI1 and RIN prolongs fruit shelf life. | Scientific Reports

Figure 5: Genetic manipulation of SlMSI1 and RIN prolongs fruit shelf life.

From: Polycomb-group protein SlMSI1 represses the expression of fruit-ripening genes to prolong shelf life in tomato

Figure 5

(A) Ripening comparison among the control, mutant rin, and SlMSI1 overexpression lines L1, L2 and L29. (B) The dehydration statistics in the transgenic and control fruits at 30, 40, and 50 days after the breaker stage. The fresh weight of the fruit at the breaker stage was recorded as the starting point. The water loss was calculated as the percentage difference between the starting weight and each subsequent measurement. (C) Ripening comparison among the control, rin mutant, SlMSI1 overexpression line L29 and recovery lines (R1, R2 and R3). The background for the recovery lines is L29. (D) The relative expression of the ripening genes in lines L29, R1, R2, R3 and the control. The same letter in the same growing season means no significant differences among three biological replicates (P < 0.05). Error bars represent SE. (E) Comparison of the fruit shelf life between the control and the recovery lines. (F) The dehydration statistic for the fruits in the control and three recovery lines. (B), breaker. Control in (A–F) indicates transgenic tomatoes carrying empty vector pBIN-GFP.

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