Figure 5: Effect of MADTP compounds on the methyltransferase and the guanylyltransferase activity of VEEV nsP1.
From: The viral capping enzyme nsP1: a novel target for the inhibition of chikungunya virus infection
(a) Effect of different concentrations of MADTP-372, MADTP-314 and MADTP-346 on the in vitro guanylylation of VEEV nsP1. The m7GMP-nsP1 complex was detected by Western blot with an anti-methyl3/7Gp antibody. (b) Dose-response curves of MADTP-314 (circles), MADTP-346 (triangles), and MADTP-372 (squares) on the methyltransferase activity of VEEV nsP1. The nsP1-MTase product (3H-methyl) GIDP was measured by a scintillation counter. The average values ± SD of two independent experiments are shown. (c) Relative nsP1 guanylylation activity (% WT) of the D34S-nsP1 mutant in the absence of compounds. (d) Relative nsP1 guanylylation activity (% untreated control) of WT and D34S VEEV nsP1 when treated with different concentrations of MADTP-372. The average values ± SD of two independent experiments are shown.
