Figure 3: Dysregulation of bile salt synthesis in animals with combined steatosis and cholestasis.

(A) Plasma total bilirubin, plasma bile salt levels, and hepatic bile salt levels. Statistical analyses shown were performed on total bile salt levels. (B) HepG2-NTCP cells treated with bile salts at 200 μmol/L in total were assayed for viability, abundance, and release of AST and LDH. (C) Hepatic mRNA of Cyp7a1 and nuclear receptors that suppress Cyp7a1. Values represent means (n = 4–6) ± SD; 1, p < 0.05 versus sham; 2, p < 0.05 versus MCD; 3, p < 0.05 versus BDL; *p < 0.05.