Figure 5: Humoral responses induced in mice by immunization with chimeric RHDV-M2 VLPs.

Sera samples from groups of mice inoculated twice with the indicated VLPs were analyzed by ELISA. Serum IgG antibody titers were measured using RHDV VLPs to detect anti-VP60 antibodies (a), or a synthetic peptide encompassing the M2e sequence (b). The GMT was calculated for each group of mice. Error bars show the standard error of the mean. In (a), statistically significant differences in anti-VP60 antibody titers, with respect to that corresponding to the VP60 group are shown as *P < 0.05, **P < 0.01, ***P < 0.001. In (b), statistically significant differences in anti-M2e antibody titters between groups are shown as*.