Figure 1 | Scientific Reports

Figure 1

From: Caveolae-mediated albumin transcytosis is enhanced in dengue-infected human endothelial cells: A model of vascular leakage in dengue hemorrhagic fever

Figure 1

Enhanced internalization of BSA into DENV2-infected human endothelial cells.

(A) Intracellular BSA in DENV2-infected EA.hy926 cells at various post-infection time-points was determined by Western blot analysis. GAPDH served as the loading control while non-structural 1 (NS1) was a marker for DENV infection. (B) Quantitative analysis of BSA band intensity (C) Detection of BSA conjugated with Alexa Fluor 488 (Alexa488-BSA) (shown in green) by a laser scanning confocal microscope (LSM 510 Meta, Carl Zeiss). Hoechst staining was performed to demonstrate nuclei. DENV stained in red served as the marker for successful DENV2 infection. Original magnification was 630X for all panels. (D) Fluorescence intensity of the internalized Alexa488-BSA was quantitated using ImageJ software. (n = 3 independent experiments for both Western blot analysis and fluorescence study; *p < 0.05 vs. mock control; #p < 0.01 vs. mock control).

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