Figure 4

Effect of inhibitors of individual endocytic pathways on albumin transcytosis in DENV2-infected EA.hy926 cells.

At 47 h post-infection, the inhibitor (nystatin, chlorpromazine or EIPA) was pretreated with the cells for 10 min prior to incubation with Alexa488-BSA for 50 min. (A) The internalized Alexa488-BSA (shown in green) was imaged by a laser scanning confocal microscope (LSM 510 Meta, Carl Zeiss) (original magnification was 630X). (B) The intensity of the internalized Alexa488-BSA was quantitatively by using Axiovision software (n = 3 independent experiments; *p < 0.0005; **p < 0.0001; NS = not significant). (C) Immunofluorescence co-staining of Alexa488-BSA (in green) and caveolin-1 (in red). The images were captured by a laser scanning confocal microscope (LSM 510 Meta, Carl Zeiss) (Hoechst staining was performed to demonstrate nuclei) (original magnification was 630X).